Fig. 7

Senescence assay with non-differentiated basal cells. (A) SA-β-gal activity, (B) metabolic activity, (C) release of LDH, and (D) relative concentrations of GDF-15 and IL-8 in basal cell supernatants after treatment with Dox, Quer and Das and the combination of Dox and Quer or Das for 48 h. Each data point represents an independent experiment using cells from a separate donor. Data were compared by one-way ANOVA with Tukey’s multiple comparisons post-test and are shown as the mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001