Fig. 4

scRNA-seq analysis reveals distinct epithelial cell populations. (A–D) Organoids were pre-treated with Dox or control medium for 48 h. The organoids pre-incubated with control medium were treated with control medium or Quer, the organoids pre-incubated with Dox were treated with Dox or the combination of Quer and Dox for additional 48 h. (A) UMAP visualization of major epithelial cell types. (B) Dot plot showing expression of epithelial cell type markers. (C) UMAP visualization of cell markers for senescence. (D) Bar plot of Hallmark gene set enrichment analysis (KEGG_2019_Human, two-sided Wilcoxon Rank Sum) for pathways enriched in senescent cells. (E) RNA velocity analysis of scRNA-seq data. (F) UMAP visualization of major epithelial cell types of combined data sets