Fig. 3

Quercetin decreases SA-β-gal activity after senescence has been induced. (A) Schema of the experimental protocol. Organoids were pre-treated with Dox or control medium for 48 h. The organoids pre-incubated with control medium were treated with control medium or Quer, the organoids pre-incubated with Dox were treated with Dox or the combination of Quer and Dox for additional 48 h. (B) SA-β-gal activity, (C) representative phase contrast images of organoids (scale bar = 50 μm), and (D) percentage of organoids with visible lumen. (E) Concentrations of GDF-15 and IL-8 in supernatants. Presented data are representative for two independent experiments. Data were compared by one-way ANOVA with Tukey’s multiple comparisons post-test and are shown as the mean ± SD. **p < 0.01 and ***p < 0.001