Fig. 2

Quercetin and dasatinib counteract doxorubicin-induced senescence. (A) SA-β-gal activity, (B) representative phase contrast images of organoids (scale bar = 100 μm), (C) percentage of organoids with visible lumen, (D) metabolic activity, (E) release of LDH, and (F) release of GDF-15 and IL-8 after 48 h treatment with Dox and the combination of Dox with Quer or Das (relative to control). Each data point represents an independent experiment using cells from a separate donor. Data were compared by one-way ANOVA with Tukey’s multiple comparisons post-test and are shown as the mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001