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Fig. 1 | Respiratory Research

Fig. 1

From: Biochemical and transcriptomic evaluation of a 3D lung organoid platform for pre-clinical testing of active substances targeting senescence

Fig. 1

Doxorubicin induces a senescence-like phenotype in 3D bronchial organoids. (A) Representative phase contrast images of organoids (scale bar = 100 μm), (B) percentage of organoids with visible lumen, and (C) senescence associated β-galactosidase (SA-β-gal) activity 48 h after treatment with doxorubicin (Dox), quercetin (Quer), and dasatinib (Das). Each data point represents an independent experiment using cells from a separate donor. Data were compared by one-way ANOVA with Tukey’s multiple comparisons post-test and are shown as the mean ± SEM. *p < 0.05 (D) Fluorescence microscopy of organoids 96 h after treatment with Dox (BF = brightfield, scale bar = 50 μm, * indicate SA-β-gal activity)

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Respiratory Research

ISSN: 1465-993X

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