Fig. 5
From: Tuftelin1 drives experimental pulmonary fibrosis progression by facilitating stress fiber assembly
TUFT1 interacted with N-WASP and affected the expression of pY256N-WASP. a N-WASP and TUFT1 were co-localized in MRC5 cells detected by immunofluorescent staining, scale bars: 10 µm. b Exogenously expressed N-WASP and TUFT1 co-immunoprecipitated in HEK293T cells. c Exogenously TUFT1 and N-WASP have fine co-immunoprecipitated in HEK293T cells. d TUFT1 can induce a high expression of pY256N-WASP in MRC5 cells. e Silencing the TUFT1 could make the pY256N-WASP keep away from the nucleus in MRC5 cells compared to the control detected by immunofluorescent staining, scale bars: 20 µm. f The distance of pY256N-WASP to the core of the nucleus was quantified