Fig. 2

TUFT1 disruption inhibited bleomycin-induced mouse pulmonary fibrosis in vivo. a A schematic diagram illustrated the time and course of the experiments. b The pulmonary images of mice were detected by Micro-CT scan on the 14th day. c Representative images of H&E staining, Masson’s trichrome staining of the lung section, and Immunohistochemical staining images of α–SMA, scale bars: 200 µm. d Ashcroft score (mean ± SD). e Quantitative hydroxyproline assay of the right lung (mean ± SD, n = 8 mice per group). f Western blotting assay of FN, collagen I, and Tuft1 in the lung homogenate; β-actin used as the loading control. g-i Corresponding optical densitometry analysis of (f) (mean ± SD). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001