Table 1 Antibodies used for staining in flow cytometric analysis and immunoblot
Target | Clone | Fluorochrome | Manufacturer |
|---|---|---|---|
CD45 (flow cytometry) | 30-F11 | PerCP-Cy5.5 | BioLegend |
Ly6G | 1A8 | APC | BioLegend |
CD11b | M1/70 | APC-Cy7 | BioLegend |
CD11c | N418 | Brilliant Violet 421 | BioLegend |
Siglec F | E50-2440 | PE-CF594 | BD Biosciences |
CD24 | M1/69 | Brilliant Violet 650 | BD Biosciences |
CD103 | 2E7 | PE | BioLegend |
MHC II (I-A/I-E) | M5/114.15.2 | PE-Cy5 | BioLegend |
Ly6C | HK1.4 | Alexa Fluor 700 | BioLegend |
CD206 | C068C2 | PE-Cy7 | BioLegend |
Arginase | polyclonal | PE | R&D Systems |
Lyn | C13F9 | none | Cell Signaling |
Phospho-Lyn (Tyr397) | MA5-35,882 | none | Thermo Fisher |
CD36 (immunoblot) | EPR6573 | none | Abcam |
CD36 (immunofluorescence) | JJ2005 | none | invitrogen |
CD45 (immunofluorescence) | 30-F11 | BV421 | BD Biosciences |
Anti-rabbit IgG | Alexa Fluor® 647 | Cell signaling |