Fig. 7

CD36-dependent TGFβ production in response to oxPL is mediated by Lyn kinase. A Lysate from bone marrow derived macrophages (BMM) treated with 100 µM SSO (vs. DMSO vehicle control) and oxPL (POVPC at 10 µg/ml) were immunoprecipitated for CD36 and analyzed by immunoblot for CD36 and Lyn kinase. B WT BMMs treated with 100 µM SSO (vs. DMSO vehicle control) and CD36-null BMM were treated with oxPL (POVPC at 10 µg/ml) were analyzed for levels of Lyn and phosphorylated Lyn (p-Lyn). C TGFβ ELISA of conditioned media from WT BMMs treated with 10 µM Bafetinib (vs. DMSO vehicle control) and oxPL. N = 4–6 per group,*p < 0.01 compared to BMMS treated with DMSO and oxPL. D, E Four days after WT and CD36-null mice were injured with POVPC (or PBS control) lysate from lungs (D) or BAL cells (E) were analyzed for Lyn and p-Lyn by immunoblot. Images shown are cropped from the the original image files