Fig. 3

Lipid uptake by BAL cells and BAL TGFβ concentration are regulated by CD36. A, B Four days after intrapulmonary delivery of oxPL (POVPC at 10 µg/gram) lung sections were visualized by H&E staining (200x). WT mice (A) develop alveolar accumulation of large lipid laden foamy macrophages (arrows). CD36-null mice have less accumulation of foamy macrophages (B). C Lipid accumulation determined by nile red staining of BAL cells from WT and CD36-null mice at several timepoints after treatment with PBS or oxPL (POVPC 10 µg/gram). N = 8–12 per group. *p < 0.01 compared to WT mice 4 days after oxPL. **p < 0.01 compared to WT mice 10 days after oxPL. ***p < 0.05 compared to uninjured WT mice. D Representative images (400x) of nile red stained BAL cells from uninjured WT and CD36-null mice 10 days after oxPL injury