Fig. 1
From: An epithelial gene signature of trans-IL-6 signaling defines a subgroup of type 2-low asthma
sIL-6R amplifies activation of the IL-6 pathway signaling and amplifies gene regulation in the airway epithelial cells. A Western blot analysis of BEAS-2B cells stimulated for 15 min solely or in combination with 10 ng/mL IL-6, 100 ng/mL sIL-6Rα, or a combination of both IL-6 and sIL-6Rα. Western blots were stained for pSTAT3 and quantified. Western blots represent six experiments. Statistically significant differences are indicated *p < 0.05; ****p < 0.0001. B Normalized TEER and C, xCELLigence show no effect of IL-6 and sIL-6Rα on the integrity of the epithelial barrier after and during 24 h of stimulation respectively. Differential gene expression analysis of D IL-6 vs. control and E IL-6 + sIL-6Rα vs control