Fig. 7
From: CSE triggers ferroptosis via SIRT4-mediated GNPAT deacetylation in the pathogenesis of COPD
Experimental validation of CSE-induced ferroptosis-related markers via SIRT4-mediated GNPAT acetylation. (A) Detection of lipid oxidation by the BODIPY 581/591 C11 probe method. (B) Mitochondrial morphology associated with ferroptosis was determined by transmission electron microscopy. (C) Content detection of the ferroptosis-related markers MDA, GSH, and GPX4 by ELISA. Data are presented as the mean ± SD of three replicates and analyzed using ANOVA, followed by Tukey’s post-hoc test. ***p < 0.001, compared with blank + vector; ###p < 0.001, compared with 5% CSE + vector; $$p < 0.01, $$$p < 0.001, compared with 5% CSE + SIRT4