Fig. 1

Investigation of the association between the expression of plasmalogen biosynthesis and ferroptosis in COPD mice. (A) H&E staining of sections of mouse lung tissues from COPD model and normal group. (B) The concentration of IL-33 and IL-1α in the bronchoalveolar lavage fluid was determined by ELISA assay. (C) The neutrophils, macrophages, and lymphocytes in bronchoalveolar lavage fluid were quantified and compared between COPD and normal groups. (D) Tissue damage of lung were elevated by immunohistochemistry staingin of Bax. (E) GPX4 activity and the contents of GSH and MDA were analyzed between COPD and normal groups. (F) GPX4 expression in the lung tissues of mice was tested by immunohistochemistry (×100 and ×200). (G) The protein expression of GPX4, FAR-1, AGPS, and GNPAT was detected via western blot analysis. (H) The mRNA expression of FAR-1, AGPS, and GNPAT was measured via quantitative real-time PCR. (I) GNPAT expression in the lung tissues of mice was tested by immunohistochemistry (×100 and ×200). Data are presented as the mean ± SD of three replicates and analyzed using Student’s t-test. ^nsp > 0.05, **p < 0.01, ***p < 0.001, compared with normal. The gels were cropped reasonably