Fig. 6
From: Age-dependent inflammatory response is altered in an ex vivo model of bacterial pneumonia
Neutrophil surface markers are age-dependently regulated upon P. aeruginosa exposure. Neutrophils of young and old mice were left unstimulated (Ctrl) or stimulated with bacteria (PAO1 or D61) at MOI of 5 for 4 h. A Receptor expression of CD11b, CD16, CD32, and CD88 was analyzed using flow cytometry and is depicted as mean fluorescence intensity (MFI). B Secretion of TNF-α and IL-1β was analyzed in culture supernatants. n = 6 mice per age group. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with respective control within one age group. # p < 0.05, ## p < 0.01, between the two age groups. $ p < 0.05, $$$ p < 0.001, between two bacterial strains within one age group. All other conditions are not significant