Fig. 5
From: Age-dependent inflammatory response is altered in an ex vivo model of bacterial pneumonia
NET formation is lower in response to the clinical P. aeruginosa isolate and differs morphologically. Neutrophils of young and old mice were left unstimulated (Ctrl), stimulated with a PMA/ionomycin cocktail, PMA/ionomycin plus DNase I or bacteria (PAO1 or D61) at MOI of 5 for 4 h. (A) NET formation was monitored by adding SYTOX green and measurement of relative fluorescence units (RFU). SYTOX green signal was normalized to unstimulated control neutrophils (Ctrl) to yield the fold change compared with Ctrl. n = 6 mice per age group. *** p < 0.001, compared with respective control within one age group. $ p < 0.05, between two bacterial strains within one age group. All other conditions not significant. No differences between the two age groups. (B) NET formation was investigated using scanning electron microscopy. Representative images are shown for neutrophils of young (A-C) and old mice (D-F), scale bar = 10 μm. NET formation of neutrophils from young mice in response to PAO1 (G) or D61 (H) is shown as a close-up image, scale bar = 2 μm