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Fig. 4 | Respiratory Research

Fig. 4

From: METTL3-mediated m6A RNA methylation induces the differentiation of lung resident mesenchymal stem cells into myofibroblasts via the miR-21/PTEN pathway

Fig. 4

METTL3-mediated m6A RNA methylation promotes pri-miR-21 processing by DGCR8. A Prediction score distribution of the potential m6A modification sites along the pri-miR-21 sequence. B RIP-qPCR analysis of pri-miR-21 enrichment by METTL3, m6A and DGCR8 in LR-MSCs with or without METTL3 silencing. (C-D) qRT‒PCR analysis of pri-miR-21 (C) and miR-21 (D) expression levels in the METTL3 overexpression group or METTL3 silencing group compared to the control. E, F qRT‒PCR analysis of pri-miR-21 (E) and miR-21 (F) expression levels in the following four cases: control group, METTL3 silencing group, TGF-β1-treated group, and METTL3 silencing group treated with TGF-β1. U6 and β-Actin were used as reference genes. **P < 0.01

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Respiratory Research

ISSN: 1465-993X

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