Fig. 3

METTL3-mediated m6A RNA methylation is involved in TGF-β1–induced pulmonary fibrosis in the murine lung. A, B The m6A levels (arbitrary units) in mouse fibrotic lungs relative to normal lungs. C, D The mRNA and protein expression levels of METTL3 in mouse fibrotic lungs relative to normal lungs by qRT‒PCR and WB assays. E HE staining of lung tissues from mice exposed to bleomycin with or without METTL3 silencing. F The α-SMA, type I collagen, and Vimentin expression levels in lung tissues from mice exposed to bleomycin with or without METTL3 silencing. β-Actin was used as a reference gene. G, H The m6A levels and METTL3 levels in primary LR-MSCs isolated from mouse fibrotic lungs and normal lungs. *P < 0.05, **P < 0.01