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Fig. 2 | Respiratory Research

Fig. 2

From: METTL3-mediated m6A RNA methylation induces the differentiation of lung resident mesenchymal stem cells into myofibroblasts via the miR-21/PTEN pathway

Fig. 2

TGF-β1-induced differentiation of LR-MSCs into myofibroblasts is dependent on METTL3-mediated m6A RNA methylation. A Relative m6A levels (arbitrary units) of LR-MSCs at baseline and after TGF-β1 stimulation. B, C The mRNA and protein expression levels of METTL3, METTL14, WTAP, FTO, and ALKBH5 in LR-MSCs at baseline and after TGF-β1 stimulation by qPCR and WB assays. D Relative m6A levels (arbitrary units) of LR-MSCs in the following four cases: control group, METTL3 silencing group, TGF-β1-treated group, and METTL3 silencing group treated with TGF-β1. E, G The mRNA and protein expression levels of myofibroblast markers (α-SMA, type I collagen, and vimentin) in LR-MSCs treated as described above. β-Actin was used as a reference gene. Scale bars = 20 μm, **P < 0.01

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Respiratory Research

ISSN: 1465-993X

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