Fig. 3
From: Characterization and inhibition of inflammasome responses in severe and non-severe asthma
Increased LPS-induced NLRP3 inflammasome priming and nigercin-induced activation in peripheral blood mononuclear cells (PBMCs) from patients with severe asthma compared to other subjects is not affected by sex or obesity. PBMCs from patients with non-severe (NSA) and severe stable (SA(S)) asthma, and healthy (H) subjects were pre-stimulated with LPS for 4 h before stimulation with nigericin for 1 h, and the effects of LPS + Nigericin on IL-1β release was assessed in culture supernatants. Some cells were treated with MCC950 at the same time as nigericin stimulation. The effects of LPS + Nigericin stimulation and NLRP3 inflammasome inhibition on IL-1β release were assessed in patients with SA(S) compared to other subjects (H and NSA together) following stratification of subjects by sex A and obesity B status. Data are presented as means ± SEM (N = 15–70). *P < 0.05, **P < 0.01. #P < 0.05 compared to control not treated with MCC950