Fig. 2
From: Characterization and inhibition of inflammasome responses in severe and non-severe asthma
Nigericin-induced NLRP3 inflammasome-mediated IL-1β release in peripheral blood mononuclear cells (PBMCs) from patients with asthma is not affected by sex or obesity. PBMCs from patients non-severe (NSA) and severe stable (SA(S)) asthma and healthy (H) subjects were pre-cultured with media for 4 h before being stimulated with nigericin for 1 h, and the effects on IL-1β release assessed in culture supernatants. Some cells were treated with MCC950 at the same time as nigericin stimulation. The effects of nigericin stimulation and NLRP3 inflammasome inhibition on IL-1β release were assessed following stratification of subjects by sex A and obesity ) status. Data are presented as means ± SEM (N = 11–59). **P < 0.01, ***P < 0.001. #P < 0.05 compared to control not treated with MCC950