Fig. 7

Blocking ENO1 reduces plasmin activation and collagen secretion in primary human lung fibroblasts. (A) DHLF-IPF was allowed to grow for 48 h in the absence or presence of indicated concentrations of HL217, hIgG1, or TXA. Untreated NHLF was cultured as control. The cell culture supernatants were collected and subjected to the measurement of collagen by using Sircol assay. (B) Primary normal human lung myofibroblasts (NHLF-Myo) were differentiated from NHLF by treatment with TGF-β for 72 h. Increasing expression of fibronectin and α-SMA confirmed the differentiation of NHLF-Myo. Cropped blots were shown, and supplementary Fig. S7 presented the full-length blots. (C) The NHLF-Myo was treated with TGF-β for another 4 h and subjected to the measurement of cell-associated plasmin activity in the absence or presence of indicated concentrations of HL217, hIgG1, or TXA. (D) The cell culture supernatants were collected and subjected to the measurement of collagen by using Sircol assay. ^#P < 0.05, ^###P < 0.001 vs. the untreated group; ^&&P < 0.01 vs. the NHLF group; *P < 0.05, **P < 0.01 vs. the TGF-β-treated group. Pooled data of three independent experiments were shown as mean ± SD (one technical replicate and three biological repeats per group) except (B) was representative for two independent experiments (each protein band was from one biological repeat)