Fig. 4

Anti-inflammatory effects of ENO1 Ab HL217 in LPS-stimulated primary human PBMC. Fresh peripheral blood was collected from 3 healthy donors and added with indicated concentrations of LPS, control human IgG1 (hIgG1), and HL217 for 4 h, followed by isolation of PBMC. The isolated PBMC was subjected to measurement of cell-associated plasmin activity (A) and cell migration (B). (C-F) PBMC was isolated from 3 healthy donors and treated with indicated concentrations of LPS, hIgG1, and HL217 for 24 h. Cell culture supernatants were collected and subjected to measurement of pro-inflammatory cytokines, including TNF-α (C), IL-1β (D), IL-6 (E), and CCL2 (F), by ELISA. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. untreated cells; *P < 0.05, **P < 0.01, ***P < 0.001 vs. the LPS-treated group. Pooled data of three independent experiments and each data point was from one human subject