Fig. 2
ISM1 deficiency exacerbates HDM-induced allergic asthma phenotypes. (A) Sensitization protocol of HDM-induced asthma in WT and ISM1−/− mice. Animals were sensitized on days 0, 7, and 14 with 50 µg of HDM extract and euthanized on day 17. (B) Serum titer for total and HDM-specific IgE comparing WT (n = 6) and ISM1−/− (n = 8) mice with or without HDM sensitization. (C) Cellular composition of BALF measured by flow cytometry (n = 13). (D-E) Representative photomicrographs of H&E staining of lung tissue sections along with the immune cell infiltration score between WT (n = 5) and ISM1−/− (n = 5) mice with or without HDM sensitization. Bars = 50 μm. (F-G) Representative photomicrographs of PAS staining of the lung tissue section along with the PAS-positive cell score between WT (n = 5) and ISM1−/− (n = 5) mice with or without HDM sensitization. Bars = 50 μm. (H) Total TGFβ1 levels in the BALF quantified using ELISA (n = 5). (I) Western blot analysis of cleaved TGFβ1 expression in lung tissues (n = 5). Data are presented as the mean ± SD. **P < 0.01; ***P < 0.001; ****P < 0.0001, using one-way ANOVA. H&E, hematoxylin and eosin; PAS, Periodic acid-Schiff, TGFβ1, transforming growth factor beta 1