Fig. 6

Flavored e-cig dysregulated EMT in lung bronchial epithelium

BEAS-2B cells exposed to air, PG/VG, and tobacco flavored e-cig for 10 min, and then cultured for 2 days. (A) Cells were lysed and RNA was isolated. The gene expression levels of CDH1, CDH2, OCLN, VIM, TJP1, and SERPINE1 were measured by qRT-PCR, and GAPDH was used as the endogenous control. (B) Protein was isolated and expression levels of Occludin, ZO-1, Vimentin, N-cadherin, PAI-1, and E-cadherin were measured by western blot. GAPDH was used as the endogenous control for both RNA and protein normalization. Data presented as mean ± SEM. (n = 5–6. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. air)