Fig. 6

Effect of JQ1 on TNFα stimulated induction of IL-6 and IL-8 mRNA levels in HPASMC. HPASMCs from control (lefthand panels) and PAH (righthand panels) patients were pre-incubated with JQ1 ± (1000 nM) for 2 h prior to the addition of TNFα (10 ng/ml) for 4 h. Cells were collected, RNA extracted, after which cDNA synthesis and RT-PCR was performed for IL-6 A and B and IL-8 C and D. Data are shown as mean ± SEM of N = 8 (4 Donors at 2 different passages) independent experiments. Statistical comparisons were made using Kruskal-Wallis one-way ANOVA with Dunns post-test. *p < 0.05, **p < 0.01, ***p < 0.001 when comparing to media control or as indicated by the line