Fig. 4

Effect of JQ1 on TNF stimulated enrichment of NF-κB p65 and BRD4 to -κB binding sites on IL-6 and IL-8 promotors in HPMEC. HPMEC from control (lefthand panels) and PAH (righthand panels) patients were pre-incubated with JQ1 ± (1000 nM) for 2 h prior to treatment with TNFα (10 ng/ml) for 3 h. Cells were harvested and ChIP analysis performed to determine enrichment of NF-κB p65 to κB binding sites on IL-6 A and B and IL-8 C and D promotors. Additionally, enrichment of BRD4 to -κB binding sites on IL-6 E and F and IL-8 G and H promotors was determined. Data are shown as mean ± SEM of N = 8 (4 Donors at 2 different passages) experiments. Statistical comparisons were made using Kruskal-Wallis one-way ANOVA with Dunns post-test. *p < 0.05, **p < 0.01, ***p < 0.001 when comparing to media control or as indicated by the line