Fig. 1

BRD2 and 4 protein expression in vascular cells from PAH patients and controls. Immunohistochemistry was performed on lung tissue sections from control (n = 12) and PAH (n = 14) patients to determine levels of BRD2 and 4 protein expression. A BRD2 expression in pulmonary endothelial cells from PAH patients (a) and controls (b) and smooth muscle cells from PAH patients (c) and controls (d). B BRD4 expression in pulmonary endothelial cells from PAH patients (a) and controls (b) and smooth muscle cells from PAH patients (c) and controls (d). × 100 magnification, bar = 20 μm. Slides were scored and average BRD2 expression in the nucleus and cytosol of pulmonary endothelial cells C and D and smooth muscle cells E and F plotted. BRD4 expression in the nucleus of pulmonary endothelial cells G and smooth muscle cells H. Data are shown as scatter plots with mean ± SEM. Statistical comparisons were made using non-parametric t-test **p < 0.001, ***p < 0.0001