Fig. 4
From: CFTR dysfunction in smooth muscle drives TGFβ dependent airway hyperreactivity
Cftrfl/fl; SM-Cre mice treated with TGFβ had no significant goblet cell hyperplasia or alterations in ASM morphology. A. Trichrome staining revealed epithelial hyperplasia, increased inflammatory infiltrates around airways, and thickened basement membranes in Ad-TGFβ exposed mice of both genotypes. B. Although there was a trend towards increased percentage goblet cells after TGFβ exposure, as determined by morphometric analysis of PAS staining, neither control Cftrfl/fl mice nor Cftrfl/fl; SM-Cre mice demonstrated significant goblet cell hyperplasia. Arrows indicate PAS positive cells. C. Morphometric analysis of αSMA stained lung sections showed that Ad-TGFβ exposure did not alter ASM area in either control Cftrfl/fl or Cftrfl/fl; SM-Cre mice. Data are presented as mean ± SD