Fig. 3
From: CFTR dysfunction in smooth muscle drives TGFβ dependent airway hyperreactivity
After pulmonary TGFβ exposure, Cftrfl/fl; SM-Cre mice demonstrated similar changes in pulmonary TGFβ, weight, and inflammation. A. Total TGFβ levels, as determined by ELISA, were significantly increased in BAL fluid three days after pulmonary Adenoviral (Ad)-transforming growth factor (TGF) β exposure. *P < 0.05 by Mann-Whitney test. B. Treatment with Ad-TGFβ induced weight loss at day 3 in both control Cftrfl/fl mice and Cftrfl/fl; SM-Cre mice. *P < 0.05 versus respective empty vector control mice of the same genotype by two-tailed t-test. C. Both Cftrfl/fl and Cftrfl/fl; SM-Cre mice demonstrated a trend toward increased total BAL cell count after Ad-TGFβ treatment, although this did not reach significance. D. Ad-TGFβ treatment caused similar changes in differential cell count of BALF in control Cftrfl/fl mice and Cftrfl/fl; SM-Cre mice, with decreased percentage macrophages and increased percent lymphocytes and neutrophils. *P < 0.05 versus respective empty vector control mice of the same genotype by two-tailed t-test. Data are presented as mean ± SD