Fig. 8

Histo-pathology results of the lungs in PH established untreated and PH established Anc.80AC treated animals. Aa–Af Representative hematoxylin–eosin and Verhoeff-van Gieson staining. Aa, Ab Sham animals; normal pulmonary arteries. Ac, Ad PH.Anc80.Null. Concentric laminar neointimal lesions. A cross-sectional view of completely occluded small pulmonary arteries by a complex lesions (grade 3–4). Ae, Af PH.Anc.80.AC. Mild neointimal reaction and proliferation, recanalization of small pulmonary artery (grade 1–2). B, C Percentage of occluded small pulmonary vessels, and medial wall thickness post Anc.80AC gene therapy in rats 8 weeks post-PH development. Scale bar: 100 μm. All continuous data were checked for normality and are presented as mean ± SD. Each group included 8 rats. Not significant p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001