Fig. 7

Assessment of main ceramides after Anc.80AC gene therapy in rats 8 weeks post-PH development. A–C Main ceramides level changes in Sham, PH.Anc80.Null and PH.Anc80.AC groups (pg/gr protein). AC gene therapy decreased level of main ceramide types. All continuous data were checked for normality and are presented as mean ± SD. Each group included 8 rats. Not significant p > 0.05; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001. D Representative western blot imaging of main senescence markers in lungs after Anc.80AC gene therapy in rats 8 weeks post-PAH creation. Senescence markers expression decreased after AC gene therapy. P21 cyclin-dependent kinase inhibitor 1, MMP-3 metalloproteinase-3, kDa kilodaltons, low molecular weight proteins