Fig. 8

Delayed activation of AMPK reduces AECs apoptosis in PAK-infected mice. (a) Representative images depicted fluorescence patterns of apoptotic cells (TUNEL) and AECs (T-1α) in the lung sections from indicted groups of mice, T-1α (green), TUNEL (red), nucleus (blue). Scale bar 100 μm. (b) Quantitative analysis of apoptotic cells. Scatter dot plot with data presented as fold control (untreated), mean ± s.d., n = 4–14 images/per indicated groups of mice. *P < 0.05 (ANOVA). (c,d) The effects of metformin and Tideglusib on macrophage-dependent continuous clearance of apoptotic AECs. (c) Representative flow cytometry charts, and (d) quantitative analysis of apoptotic AECs uptake by macrophages, ex vivo. Macrophages received dose one of apoptotic cells for 4 h and then a second dose for an additional 4 h. Representative flow charts (gating strategy) are showed along with Box plot. Data are presented as fold changes of control for n = 6 independent samples/group. *P < 0.05 (ANOVA)