Fig. 5

PAK-induces ER-stress in AECs is reduced upon therapeutic administration of AMPK activators. (a) Representative images show fluorescence patterns of CHOP (red), T-1α (green; AECs Type 1 marker) and nuclei (blue) in lung sections from control and mice exposed to PAK for 72 h. Metformin (Met) or Tideglusib (Tide) were administered 24 h post PAK infection and lung sections obtained 48 h afterwards. Scale bar 100 μm. (b) Scattergrams indicate correlation of nuclei/CHOP fluorescence intensity (A.U.; arbitrary units) for corresponding images in (a). (c) Quantitative analysis of CHOP fluorescence intensity in lung sections from indicated groups of mice. Data presented as Scattered plot with fold changes of control (untreated) mice, mean ± s.d., n = 7 images/per indicated groups of mice. *P < 0.05 (ANOVA)