Fig. 7

CircItgb5 interacts with the Uba1 protein. A CHO cells transfected with circItgb5-MS2 plasmid can specifically pull-down Uba1 protein using silver staining. Strips of Uba1 protein were highlighted with red frame. B CircItgb5 RT-PCR products were determined by agarose gel electrophoresis using anti-Uba1 antibody. C The PPI network was used to analyze proteins that interacted with Uba1; the red circle represents Ube2n (E2) and Mdm2 (E3). PPI, protein–protein interaction; E, enzyme. D, E Protein level of ACE2, Mdm2, Ube2n, and Uba1 was detected in PASMCs. *0.01 ≤ P ≤ 0.05 versus Lsh-NC, **0.001 ≤ P ≤ 0.009 versus Lsh-NC. F PASMCs extracts were immunoprecipitated with anti-Uba1 or IgG and immunoblotted with anti-ACE2 and anti-Mdm2. Input protein was immunoblotted with antibodies against ACE2 and Mdm2. C, control; B, PDGF-BB. *0.01 ≤ P ≤ 0.05 versus Input-C, **0.001 ≤ P ≤ 0.009 versus Input-C. G mRNA expression of Uba1, Ube2n, Mdm2, and ACE2 in the lungs. *0.01 ≤ P ≤ 0.05 versus control, **0.001 ≤ P ≤ 0.009 versus control. All data are presented as means ± SD (n = 3 biological replicates)