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Fig. 4 | Respiratory Research

Fig. 4

From: CircItgb5 promotes synthetic phenotype of pulmonary artery smooth muscle cells via interacting with miR-96-5p and Uba1 in monocrotaline-induced pulmonary arterial hypertension

Fig. 4

Silencing circItgb5 inhibits synthetic phenotype of PASMCs. A RT-qPCR was conducted to detect circItgb5 level when circItgb5 was inhibited by lentiviral shRNA in vitro. *0.01 ≤ P ≤ 0.05 vs Lsh-NC. B Expression of COL1A1 and SM22α was detected by western blotting. *0.01 ≤ P ≤ 0.05 versus Lsh-NC, **0.001 ≤ P ≤ 0.009 versus Lsh-NC. C PASMCs proliferation was detected using EdU assay. *0.01 ≤ P ≤ 0.05 versus Lsh-NC. Scale bar = 100 μm. D Flow cytometric analysis for quantification of early apoptotic cells. E Transwell assay was used to detect the migration ability of PASMCs. All data are presented as means ± SD (n = 3 biological replicates)

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Respiratory Research

ISSN: 1465-993X

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