Fig. 4

Nestin regulates Plk1 phosphorylation at Thr-210 and vimentin phosphorylation at Ser-56. Ctrl and nestin KD HASM cells were treated with 10^–4 M ACh for 5 min, or left untreated (UT). Protein phosphorylation in HASM cells was determined by immunoblot analysis. Nestin KD diminishes ACh-induced Plk1 phosphorylation at Thr-210 (A) and vimentin phosphorylation at Ser-56 (B). Data are means ± SE (n = 4 batches of culture from 3 donors). ** p < 0.01