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Fig. 3 | Respiratory Research

Fig. 3

From: The intermediate filament protein nestin serves as a molecular hub for smooth muscle cytoskeletal signaling

Fig. 3

Contractile stimulation enhances nestin phosphorylation at Thr-315 and nestin/Plk1 coupling. HASM cells were treated with 10–4 M ACh for different time points, or left untreated (UT). A Nestin phosphorylation at Thr-315 was determined by immunoblot analysis. Data are means ± SE (n = 5 batches of culture from 3 donors). B ACh stimulation enhances the ratios of Plk1/immunoprecipitated nestin without affecting protein expression in input fraction. IgG serves as a control. Data are means ± SE (n = 4 batches of culture from 3 donors). *p < 0.05; ** p < 0.01

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Respiratory Research

ISSN: 1465-993X

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