Fig. 2

DGK inhibition enhances COXII expression and PGE₂ production. Human ASM cells were treated with DGK I (20 µM) in a time-dependent manner. Cells were lysed and immunoblotted for COXII and β-actin (A). Graphical representation of mean ± SEM of COXII densitometry data normalized to basal conditions (B). Supernatant collected was subjected to ELISA to assay amounts of PGE₂ (pg/ml) secreted and data were normalized to vehicle-treated condition (C). Data above are mean ± SEM (n = 3–4 distinct donors). *p < 0.05 (DMSO vs. DGK I at matched time points) using one-way-ANOVA with Bonferroni post-hoc analysis. Please note that the gel images shown are cropped from the full length images