Fig. 1

DGK inhibitor attenuation of ASM cell proliferation involves PKA. GFP- and PKI-GFP-expressing human ASM cells were pretreated with DGK I (0–20 µM) followed by PDGF (10 ng/ml) stimulation for 48 h and cell proliferation was measured by assessing total DNA. Graphical representation is of mean ± SEM (n = 6 distinct donors) of CyQuant fluorescence normalized to basal (A). Expression of GFP in ASM cells transduced with retroviral particles was confirmed by fluorescence microscopy. Representative florescence (top) and brightfield (bottom) images of two different lines are shown (B). *p < 0.05 (compared to matched basal) and ^#p < 0.05 (compared to matched PDGF) using one-way-ANOVA with Bonferroni post-hoc analysis