Fig. 6

Suppression of ER stress activation or SIAH2 induces HIPK2 expression. a protein levels of PERK, ATF4, SIAH2 and HIPK2 in lung tissues from each group were measured by immunoblotting. b Immunofluorescence of ATF4 in pulmonary arteries. ATF4 (green), α-SMA (red) and nucleus (blue) were stained in lung tissue from different groups. c Representative images of ER morphology by transmission electron microscopy. Scale bar = 200 nm. ER, endoplasmic reticulum. d Protein levels of SIAH2 and HIPK2 were determined by immunoblotting. For original blot images, see Additional file 1. *P < 0.05