Fig. 5

Inhibition of HMGB1, ER stress or SIAH2 reverse pulmonary hemodynamic parameters and vascular remodeling. Comparison of RVSP (a), mPAP (b), the ratio of RV/(LV + S) (c) in each group. d the HMGB1 serum level in different groups was detected by ELISA. e Representative images of hematoxylin and eosin (HE) staining revealed RV hypertrophy; HE staining and Eastic Van Gieson (EVG) staining of distal pulmonary arterioles reflect the medial wall thickness of pulmonary; Immunohistochemical staining of α-SMA revealed the muscularization of distal pulmonary arterioles. scale bar = 100 μm. f Quantitative analysis of the percentage of the medial wall thickness of pulmonary arteries. g Quantitative analysis of muscularization of distal pulmonary arteries. RVSP: right ventricle systolic pressure; mPAP mean pulmonary arterial pressure; RV/(LV + S): ratio of the right ventricle to left ventricle plus septum. h Protein levels of PERK, ATF4, SIAH2 and HIPK2 in lung tissues from each group were measured by immunoblotting. For original blot images, see Additional file 1. *P < 0.05