Fig. 5
From: Collaborative cross strain CC011/UncJ as a novel mouse model of T2-high, severe asthma
CC011 mice do not exhibit enhanced ILC2 responses to HDM compared to BALB/cJ mice. A Gating strategy for flow cytometric identification of ILC2s (Lin−CD45+ICOS+ST2+) from lung single cell suspensions. B, C Total number of lung ILC2s (B) from BALB/cJ and CC011 mice following three treatments with PBS or HDM (n = 5 mice per treatment). Significance of t-tests for treatment effects within strain or between HDM-exposed BALB/cJ and CC011 denoted by * for P < 0.05. C Number of IL-5+ and IL-13+ ILC2s as determined by intracellular cytokine staining following stimulation of cells with PMA and ionomycin