Fig. 7
From: A polo-like kinase inhibitor identified by computational repositioning attenuates pulmonary fibrosis
GSK461364 doesn’t inhibit the growth of lung epithelial cells. (A-C) The proliferation of murine primary fibroblasts or LA4 cells cultured in BI2536 stimulated with FGF-2 30 ng/ml or PDGF-BB 100 ng/ml was measured by a 3H thymidine incorporation assay. The primary fibroblasts or cultured LA4 cells cultured with 3 to 300 nM of BI2536 for 72 h. 3H-TdR was pulsed for the final 18 h, and the incorporation of 3H-TdR was measured. (D-F) The proliferation of murine primary fibroblasts or cultured LA4 cells cultured in GSK461364 stimulated with FGF-2 30 ng/ml or PDGF-BB 100 ng/ml was measured by a 3H thymidine incorporation assay. The proliferation of murine primary fibroblasts or cultured LA4 cells cultured with 3 nM to 300 nM of GSK461364 for 72 h. 3H-TdR was pulsed for the final 18 h, and the incorporation of 3H-TdR was measured. Data were analyzed using a one-way analysis of variance followed by Dunnett’s multiple comparison test and displayed as the mean ± SD. For the graph (A), (C), (D), (F) compared with the FGF-2 30 ng/ml group without drug administration: ** P = 0.001–0.01; *** P < 0.0001. The P values of each experiment are shown above each figure. For the graph (B) (E) compared with the PDGF-BB 100 ng/ml group without drug administration: ** P = 0.001–0.01; *** P < 0.0001. The P values of each experiment are shown above each figure