Fig. 6
From: Multi-omic assessment shows dysregulation of pulmonary and systemic immunity to e-cigarette exposure
Vaping exposure significantly changes T cell populations in spleen and blood of exposed animals. Animals were exposed to FA, PGVG or PGVG + phytol as described. At the end of the 8 week exposure, exposed animals were injected IV with anti-CD45 5 min prior to sacrifice, and spleens A–C and blood D–F were processed, stained, and analyzed by flow cytometry for number of CD45 + CD3 + CD4 + T cells (A, D), CD45 + CD3 + CD4 + CD25hi T cells (B, E), and CD45 + CD3 + CD4 + CD44hiCD62Llo T cells (C, F). T-test p values between 2 conditions indicated by line 2 experiments, p value for one way ANOVA of all 3 conditions shown below. n = 3 each for total of 6