Fig. 4

Alterations in mRNA transcripts in lungs following exposure to Ecig components PGVG and Phytol. Lungs from individual animals from each group (n = 3/gp) were frozen and RNA extracted for mRNA analysis using Fibrosis kit and Host Response kit using Nanostring technology. A Heat map comparing individual samples from the top 100 differentially expressed transcripts; B Overlay of statistically significant transcripts compiled from Host Response and Fibrosis kits using he partial least squares discriminant analysis (PLS-DA). C, D Volcano plot showing a comparison of statistically significant differentially expressed transcripts from lung of PGVG exposed C or Phytol exposed D versus filtered air control animals using results from both the Fibrosis and Host Response kits. Green indicates transcripts upregulated in PGVG C or Phytol D exposed lung relative to control. Purple indicates transcripts downregulated in PGVG C or Phytol D relative to control. For the full list of differentially expressed mRNA transcripts in Phytol versus FA and PGVG versus FA, see Table 3