Fig. 3
From: Multi-omic assessment shows dysregulation of pulmonary and systemic immunity to e-cigarette exposure
Alterations in metabolites in lungs following exposure to Ecig components PGVG and Phytol. Lungs from individual animals from each group (n = 5/gp) were frozen and metabolites extracted and analyzed for metaboites as detailed in Materials and Methods. A Heat map comparing individual samples from the top 50 metabolites; B Overlay of statistically significant metabolic species using the partial least squares discriminant analysis (PLS-DA). C, D Volcano plot showing a comparison of statistically significantly differential metabolites from lung of PGVG exposed C or Phytol exposed D versus filtered air (FA) control animals. Green indicates metabolites upregulated in PGVG C or Phytol D exposed lung relative to control. Purple indicates metabolites downregulated in PGVG C or Phytol D relative to control. C Isobutyric acid and tryptophan represent the largest increase in PGVG while proline is largest downregulation versus control. D Phytol volcano plot with acetylcholine as the most upregulated in phytol versus FA while proline is most downregulated in phytol compared to FA control. For the full list of metabolites differentially detected in Phytol versus FA and PGVG versus FA, see Table 2