Fig. 2

Alterations in lipids in lungs following exposure to Ecig components PGVG and Phytol. Lungs from individual animals from each group (n = 5/gp) were frozen and lipid extracted and analyzed for lipids as detailed in Materials and Methods. A Heat map comparing individual samples from the top 100 lipids; B Overlay of statistically significant lipid species using the partial least squares discriminant analysis (PLS-DA). C, D Volcano plot showing a comparison of statistically significantly differential lipids from lung of PGVG exposed C or Phytol exposed D versus filtered air (FA) control animals. Green indicates metabolites upregulated in PGVG C or Phytol D exposed lung relative to control. Purple indicates metabolites downregulated in PGVG C or Phytol D relative to control. For the full list of lipids differentially detected in Phytol versus FA and PGVG versus FA, see Table 1