Fig. 4

LPS administration induces lung injury and ferroptosis. A The representative H&E staining of lung tissue sections, magnification ×400; B the lung injury score analysis; the level of ferroptosis markers, C MDA, D GSH, E Fe²⁺ in lung tissue; F the mRNA levels of Cp, Slc7a11, Slc39a14 and Gpx4 in lung tissue were detected by real-time qPCR. G The Cp, Slc7a11, Slc39a14 and Gpx4 protein levels in lung tissue were detected by western blotting at 6 h; H Ultrastructural changes of mitochondria in lung tissue by transmission electron microscopy, magnification ×100,000. Data presented as mean ± standard deviation with *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 for all statistics