Fig. 5

Role of Nrf2 in AZI-ameliorating airway epithelial barrier dysfunction. HBECs were pre-incubated with or without AZI (50 μM), TBHQ (30 μM), Vc (50 μM) for 1 h, and subsequently co-incubated with 3%CSE for 24 h. A The levels of IL-6 and TNF-α in the supernatant of HBECs were analyzed by ELISA. B, C The contents of GSH and ROS, along with the activities of GCL and GS, were measured by relevant assay kits. D The contents of L-Glutamic acid and pyroglutamylglycine were measured by LC–MS. E TEER was monitored for 24 h at specified time points in HBECs. F–I Western blot analysis was performed to examine the expressions of indicated proteins in HBECs. Data shown are means ± SD, n = 6. ^#P < 0.05, ^##P < 0.01 and ^###P < 0.001 vs control group; ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 and ns means no significant difference vs CSE group