Fig. 4

Role of AZI on Nrf2 expression in HBECs and lung tissues of CS-exposed rats. A Immunofluorescence staining for Nrf2 and Keap1 was performed in HBECs. The scale bar represents 20 μm. B, D, E Western blot analysis was used to detect Nrf2 and Keap1 expressions in HBECs and lung tissues of rats. C Immunohistochemical staining for Nrf2 and Keap1 was performed in the lung tissues of rats. The scale bar represents 100 μm. Data shown are means ± SD, n = 6, with 3 replicates for each sample. ^###P < 0.001 and NS means no significant difference vs control group; ^*P < 0.05, ^***P < 0.001 and ns means no significant difference vs COPD/CSE group